Shotgun sequencing of the human transcriptome with ORF expressed sequence tags
نویسندگان
چکیده
منابع مشابه
Shotgun sequencing of the human transcriptome with ORF expressed sequence tags.
Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription-PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. Systematic, high-throughput sequencing of such products would result in an expressed sequence tag (EST) database consisting of central, generally coding regions of...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2000
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.97.7.3491